Scientist in Cambridge, MA

Summary

• The scientist will be responsible to run in vitro primary cell culture in 96-well plate format, perform serial dilutions of drug molecules, perform ELISA for cytokine analysis, and perform FACS flow cytometry analysis to look at cell populations changes and cell phenotype changes.
• Candidates with previous mRNA LNP production and characterization, and LNP antibody conjugation are preferred

What are the top 3-5 skills, experience or education required for this position? 

• In vitro primary cell culture (human PBMC, Cyno PBMC and mouse splenocyte)
• Serial dilutions of drug molecules and application of treatment using multichannel pipette
• Live/Dead and antibody staining of cells and FACS flow cytometry run on BD FACS Canto II / Fortessa / LSR II and data analysis using FlowJo software
• Good to have: mRNA LNP production and characterization
• Good to have: LNP antibody conjugation

What is a nice to have (but not required) skill, experience, education, or certification?

• 3-5 year experience level
• Masters preferred

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