Biomolecule Production Scientist in Cambridge, MA

BioMedicine Design (BMD) High-Throughput Protein Production (HTP) Group is seeking a highly motivated scientist to support the molecular biology & protein production workflows:

• DNA construct design/assembly, PCR, sample preparation for sequencing, DNA production & quantitation, mammalian cell culture & transfections, protein purification & quantitation, and automated liquid handling.
• The candidate will play a major role in a core group that consistently delivers hundreds of protein samples per week to research teams across the department.
• The candidate will be responsible for generating DNA constructs and performing high-throughput processes for macromolecule production (DNA, RNA, and protein).
• The candidate will also be responsible for updating the laboratory database and relaying data in organized presentations to project teams.

ROLE RESPONSIBILITIES

• DNA plasmid design & cloning
• Automated high throughput DNA purification
• High throughput molecular biology operations, including PCR, ligation reactions (Gibson & ITA), & DNA purification using liquid handling automation
• Sample preparation for sequencing
• DNA & RNA quantitation & QC
• Supporting transient mammalian transfections with DNA
• Organization and storage of DNA fragments/plasmids, including tracking using Genedata Biologics Software, as well as record keeping using electronic laboratory notebooks
• Close interaction with project teams
• Regular review of literature to maintain knowledge of scientific trends and industry processes, advance molecular biology and protein production methods

Hard Skills:

• PCR or molecular biology
• Open to Learn

Minimum education experience required:         

• Bachelor’s degree (0-2 years’ experience) or Associate’s degree (1-3 years’ experience) with experience in Molecular Biology, Biochemistry, Biology, Biological Engineering or a related discipline along with a basic understanding of molecular biology and protein expression.
• Not looking for Master or above

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