- Title: Research Associate
- Code: RCI-32156-1
- RequirementID: 119805
- Location: Bothell, WA 98021
- Posted Date: 07/26/2024
- Duration: 8 Months
- Salary ($): 47.73 - 49.25 per Hourly
- Name:Anand Akolkar
- Email: anand.akolkar@rangam.com
- Phone: 908-704-8843 ✖ 266
What is the minimum education experience required
- Bachelor’s degree + 4 years laboratory research experience or Master’s degree + 2 years laboratory research experience in biological sciences or related subject
Summary
- Client Research is seeking a collaborative and independent Scientist to participate in development of antibody-based oncology therapeutics.
- The Scientist will report to the Molecular and Cellular Technologies team lead within Antibody Discovery.
- Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns.
- The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.
Responsibilities
- Design, execute, troubleshoot, optimize, and interpret experiments
- Order constructs for overexpression and knockout cell line generation
- Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies
- Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization
- Perform routine cell culture maintenance and banking, working with both primary and cancer cells
- Optimize and perform flow cytometry assays to screen and characterize antibodies
- Maintain laboratory spaces, equipment, and supplies
- Keep an up-to-date and accurate electronic laboratory notebook
- Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings
- Proactively seek guidance and build knowledge relevant to the role
Required Qualifications
- Bachelor’s degree + 4 years laboratory research experience or Master’s degree + 2 years laboratory research experience in biological sciences or related subject
- Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions
- Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR
- Experience in multi-color flow cytometry
- Demonstrated ability to work independently and collaboratively, according to project needs
- Strong written and verbal communication skills, including ability to maintain a good working laboratory notebook
Preferred Qualifications
- Experience with FACS and single-cell cloning
- Basic molecular biology skills, including vector design, cloning, and PCR
- Knowledge of antibodies or antibody drug conjugates (ADCs)
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