Research Associate in Bothell, WA

What is the minimum education experience required

• Bachelor’s degree + 4 years laboratory research experience or Master’s degree + 2 years laboratory research experience in biological sciences or related subject

Summary

• Client Research is seeking a collaborative and independent Scientist to participate in development of antibody-based oncology therapeutics.
• The Scientist will report to the Molecular and Cellular Technologies team lead within Antibody Discovery.
• Their primary responsibilities will include engineering cell lines and performing flow-based assays to support antibody campaigns.
• The successful candidate will work well in a highly collaborative team environment and possess expertise in flow cytometry, mammalian cell culture, and cell engineering.

Responsibilities

• Design, execute, troubleshoot, optimize, and interpret experiments
• Order constructs for overexpression and knockout cell line generation
• Engineer cell lines using transfection, nucleofection, CRISPR/Cas9, lentiviral transduction, and other methodologies
• Evaluate target expression on engineered and endogenous cell lines using flow cytometry and molecular characterization
• Perform routine cell culture maintenance and banking, working with both primary and cancer cells
• Optimize and perform flow cytometry assays to screen and characterize antibodies
• Maintain laboratory spaces, equipment, and supplies
• Keep an up-to-date and accurate electronic laboratory notebook
• Present experimental findings and contribute to scientific discussion at group and cross-functional team meetings
• Proactively seek guidance and build knowledge relevant to the role

Required Qualifications

• Bachelor’s degree + 4 years laboratory research experience or Master’s degree + 2 years laboratory research experience in biological sciences or related subject
• Proficiency in culturing/maintaining multiple cell lines under BSL2 conditions
• Knowledge of cell engineering platforms, including transient transfection, generation of stable cell lines, and CRISPR
• Experience in multi-color flow cytometry
• Demonstrated ability to work independently and collaboratively, according to project needs
• Strong written and verbal communication skills, including ability to maintain a good working laboratory notebook

Preferred Qualifications

• Experience with FACS and single-cell cloning
• Basic molecular biology skills, including vector design, cloning, and PCR
• Knowledge of antibodies or antibody drug conjugates (ADCs)

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