Scientist in Swiftwater, PA

2 years experience in lab.

Must have hands on expereince in molecular biology techniques : DNA purification, PCR, Western blot, and SDS-PAGE techniques as well as aseptic cell culture tecnique.
Cell maintenance, transfection, infection, and harvest of viral stocks from suspension culture.

Have used DNA sequencing software like SanpGene.

Key Responsibilities

• Recombinant baculovirus generation, WVB.
• Protein Expression in baculovirus insect cells system in shake flasks.
• Cell maintenance, transfection, infection, and harvest of viral stocks from suspension culture.
• Sampling culture flasks and preparation of samples for analytical testing.
• Performing standard molecular biology tasks such as DNA purification, PCR, Western blot, and SDS-PAGE techniques.
• Preform aseptic techniques.
• Wash glassware and USP process equipment
• Design, analyze and document experiments, maintain proper records electronically and in lab notebooks.
• Effectively communicate results in group and department meetings
• Contribute to lab organization, reagent tracking and ordering.
• Ensure that work performed is conducted in a safe and compliant manner in adherence with SOPs/SWIs and protocols.
• Coordinate data capture and tracking information.
• Write and review protocols and reports.

Qualification

• BS in Biology, Molecular Biology, Chemical Engineering, Biochemical Engineering, Biochemistry, Biotechnology, or other relevant disciplines
• 0 -2 years of experience with BS degree working in academic or industry.
• Experience in baculovirus protein expression system and insect cell cultures is highly desirable.
• Hands-on experience in molecular biology techniques and DNA sequence analysis, construct design using SnapGene, or similar software is a plus.
• Ability to plan and conduct laboratory experiments, evaluate, and interpret data.
• Ability to work within team environment and multitasking.
• Strong written and verbal communication skills.
• Detail-oriented and ability to prioritize assignments.
• Ability to work with minimum supervision.
• Experience in cell culture procedures and associated aseptic techniques.
• Willing to work changing work schedules and hours as needed.
• Fluent in both written and spoken English.
• Strong experience in Microsoft Excel, Word and Power Point is important.

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