Scientist in Cambridge, MA

5-7 year experience level/or PhD

PBMC, Multi-color flow cytometry and PHD will be great

• Perform serial dilutions of drug molecules
• mRNA LNP preferred
• Master’s degree 3 to 5 years of experience
• PHD with 4 to 6 years of training experience will work
• BD FACS Canto II / Fortessa / LSR II
• PBMC, Mammalian cell culture is preferred
• Early discovery
• Primary cell culture
• Multi-color flow cytometry is a must          
• PHD candidate will be fine
• Bachelor’s degree 3 to 5 years of experience
• The relocation candidate will be fine
• There is no overqualification for this role
• Serial dilutions of drug molecules and application of treatment using multichannel pipette

Masters preferred

Summary

• The scientist will be responsible to run in vitro primary cell culture in 96-well plate format, perform serial dilutions of drug molecules, perform ELISA for cytokine analysis, and perform FACS flow cytometry analysis to look at cell populations changes and cell phenotype changes.
• Candidates with previous mRNA LNP production and characterization, and LNP antibody conjugation are preferred

What are the top 3-5 skills, experience or education required for this position? 

• In vitro primary cell culture (human PBMC, Cyno PBMC and mouse splenocyte)
• Serial dilutions of drug molecules and application of treatment using multichannel pipette
• Live/Dead and antibody staining of cells and FACS flow cytometry run on BD FACS Canto II / Fortessa / LSR II and data analysis using FlowJo software
• Good to have: mRNA LNP production and characterization
• Good to have: LNP antibody conjugation

What is a nice to have (but not required) skill, experience, education, or certification?

• 3-5 year experience level
• Masters preferred

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