Scientist in Waltham, MA

Note

• Broad scientific knowledge in liver biology and non-viral delivery and molecular biology.
• Experience with standard aseptic cell culture technique, including experience culturing various primary cells, hepatocytes primary cell culture, hepatocytes and/or liver non-parenchymal cells isolation.
• Proficiency across cellular, biochemical, and molecular techniques including protein detection (ELISA, MSD, western blot), gene profiling (RNA/DNA extraction, qPCR) and cell imaging.
• Experience with high content microscopy is a plus.
• Cell-based functional assays, including transfection, cytotoxicity, cytokine release, and proliferation assays.
• Experience with co-culture in-vitro or MPS is a plus.

Basic Qualifications

• Demonstrated expertise with standard aseptic cell culture technique, including experience culturing various primary cells.
• Experience with hepatocytes primary cell culture is highly appreciated.
• Experience with hepatocytes and/or liver non-parenchymal cells isolation is a plus.
• Broad experience with cell-based functional assays, including transfection, cytotoxicity, cytokine release, and proliferation assays. Experience with co-culture in-vitro or MPS is a plus.
• Proficiency across cellular, biochemical, and molecular techniques including protein detection (ELISA, MSD, western blot), gene profiling (RNA/DNA extraction, qPCR) and cell imaging. Experience with high content microscopy is a plus.
• Broad scientific knowledge in liver biology and non-viral delivery and molecular biology.
• Self-motivated with strong time management and organizational skills
• Flexibility to rapidly changing priorities and deadlines
• Experience in preparing high quality documents including technical reports, presentations, SOPs, and experimental protocols.
• Highly collaborative with demonstrated ability to work in a matrixed team environment.
• Excellent oral and written communication skills

Key Responsibilities:

• Maintain cultures of immortalized tumor cell lines, hepatic primary cell types (e.g. hepatocytes, LSEC and KC).
• Perform hepatocytes mouse isolation from key disease models.
• Optimize, design, execute, and interpret in vitro cell-based assays to characterize diverse mechanisms of gene therapies (e.g. ELISA, MSD, high content imaging)
• Perform fit-for-purpose in vitro and ex vivo assays to assess phenotypic and functional read-outs to assess drug candidate toxicity and efficacy.
• Prepare experimental protocols, analyze experimental data, and present study results in team and department meetings.
• Document experimental methods and findings in electronic laboratory notebook

POSITION OBJECTIVES

Conduct and interpret cell-based in vitro assays to characterize mechanism of action related to non-viral gene therapy with focus on the hepatic environment:

• Immunomodulation
• FCargo cell localization
• Support non-viral (LNP) oligo therapy screenings e.g. endosomal scape assessment

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