Research Technician / Associate Scientist in North Chicago, IL

100% on site

Position Title: Research Technician in Cell and Molecular Biology

Can be level II or III depending on experience

Length of Contract: 6 months

• A Research Technician position in Cell and Molecular Biology is available at the Genomic Research Center (GRC) at client.
• We are seeking a highly motivated research technician to work independently and collaboratively with other scientists on projects involving human-induced pluripotent stem cell (hiPSC) reprogramming, cell differentiation, CRISPR editing, high-throughput functional screening, and a variety of molecular biology experiments in the wet lab.
• The successful candidate will be responsible for performing time-sensitive experiments and supporting extended hiPSC differentiation protocols, both of which require daily attention to detail.

The applicant is expected to:

• Conduct routine experimental protocols and procedures in the wet lab.
• Maintain tissue culture, including stem cells and stem cell-derived cells.
• Accurately record experimental setups, SOP protocols, and data quality control.
• Maintain efficient and continuous communication with the manager and team members.
• Support team members with routine cell culture procedures, including media preparation and reagent aliquoting.
• Conduct molecular and cell biology experiments such as qRT-PCR, immunofluorescence, and Western blot analyses.

Qualifications

• A bachelor’s or master’s degree in biochemistry or molecular cell biology, with at least three years of hands-on laboratory experience.
• Trained and experienced in using aseptic techniques in a laminar flow biosafety cabinet.
• Significant cell culture expertise, including maintenance, freezing, thawing, and passaging.
• Proficiency in biochemical assays such as Western blot, immunofluorescence, and flow cytometry.
• Skilled in molecular biology techniques such as RNA extraction, cDNA synthesis, and RT-qPCR.
• Knowledge of serial dilution and calculation of solution concentration.
• Excellent written and oral communication skills.
• Organized in planning long experimental protocols that may sometimes require working on weekends.

Nice to have:

• Experience with culturing human iPSC and differentiating iPSC into brain cell types.

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